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Kobayashi et al (2010) Oral administration of probiotic bacteria, Lactobacillus casei and Bifidobacterium breve, does not exacerbate neurological symptoms in experimental autoimmune encephalomyelitis. I

Kobayashi et al (2010) Oral administration of probiotic bacteria, Lactobacillus casei and Bifidobacterium breve, does not exacerbate neurological symptoms in experimental autoimmune encephalomyelitis. I

Citation

Kobayashi Y, Kato I, Nanno M, Shida K, Shibuya K, Matsuoka Y, Onoue M (2010) Oral administration of probiotic bacteria, Lactobacillus casei and Bifidobacterium breve, does not exacerbate neurological symptoms in experimental autoimmune encephalomyelitis. Immunopharmacology & Immunotoxicology 32 (1): 116-124.

Objective

To investigate the safety and influence of probiotic bacteria, Lactobacillus casei Shirota (LcS) and Bifidobacterium breve Yakult (BbY) on experimental autoimmune encephalomyelitis (EAE), an animal model for human multiple sclerosis.

Methods

Two different antigens were prepared from guinea pigs and used as sensitising agents to induce EAE in Lewis rats. Three experiments were performed to test different schedules of probiotic administration. These were: (1) a homogenate of spinal chord to induce EAE in 7 weeks old male rats / LcS was given orally one week before this and for the duration of the experiment (total 35 days); (2) myelin basic protein (MBP) to induce EAE in 7 weeks old female rats / LcS given as before; (3) MBP in both sex rats/ but LcS and BbY administration started since the animals were 2 weeks old and continued until end of the experiment (total 63 days). Body weight, neurological symptoms and MBP-specific IgG responses were observed.

Results

No significant differences were seen in neurological symptom severity, weight loss, development of EAE orMBP-specific IgG response between control and probiotic rats in any of the experiments. In test (1) a trend was observed for LcS to suppress the development of neurological symptoms.

Conclusions

The authors concluded that the results support the hypothesis that LcS and BbY do not exacerbate autoimmune diseases.

 
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