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De Preter et al (2008) Effect of dietary intervention with different pre- and probiotics on intestinal bacterial enzyme activities

De Preter et al (2008) Effect of dietary intervention with different pre- and probiotics on intestinal bacterial enzyme activities

Citation

De Preter V, Raemen H, Cloetens L, Houben E, Rutgeerts P, Verbeke K (2008). Effect of dietary intervention with different pre- and probiotics on intestinal bacterial enzyme activities. European Journal of Clinical Nutrition 62: 225-231

Objective

Glycosides in the gut, which come from the diet or excreted in bile from the liver, can be hydrolysed by gut bacteria to form products that are potentially toxic or carcinogenic.  To investigate if this metabolism could be suppressed, this study examined the effect of probiotics and/or prebiotics on the activity of two enzymes: β-glucuronidase and β-glucosidase.

Methods

In a placebo-controlled, crossover study 53 subjects were randomised to five different interventions (see table 1). Each group had three or four treatment periods of four weeks, during which a prebiotic, probiotic, synbiotic combinations or placebo were consumed twice daily. Intervention periods were separated by either a three day (groups 1, 2 and 3) or two week washout period (groups 4 and 5). Test substances were oligofructose-enriched inulin (OF-IN), lactulose, Lactobacillus casei Shirota (LcS), Bifidobacterium breve Yakult (BbY) and a Saccharomyces boulardii preparation (Sb).

The effects of these interventions was assessed by spectrophotometric analysis of enzyme activity (b-glucuronidase and b-glucosidase) in faecal samples collected over a three day period, at baseline and after each treatment and washout period.

Results

A significant reduction of β-glucuronidase activity was observed associated with prebiotic intervention; a reduced trend was observed with LcS and BbY but there was no effect with the yeast.

LcS and Sb did not influence b-glucosidase activity, whereas a significant increase in activity was evident with BbY (P = 0.015). A non-significant reduction in activity was seen after prebiotic consumption.

Both synbiotic combinations showed a decrease in bacterial enzyme activities, although not to significance; and did not have advantages over either of the substances when administrated alone.

Conclusions

Consumption of either lactulose, OF-IN, LcS or BbY resulted in reduced activity of the bacterial enzyme β-glucuronidase in faecal samples.

 
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