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De Preter et al (2007) Effects of Lactobacillus casei Shirota, Bifidobacterium breve and oligofructose-enriched inulin on colonic nitrogen-protein metabolism in healthy humans.

De Preter et al (2007) Effects of Lactobacillus casei Shirota, Bifidobacterium breve and oligofructose-enriched inulin on colonic nitrogen-protein metabolism in healthy humans.

Citation

De Preter V, Vanhoutte T, Huys G, Swings J, de Vuyst L, Rutgeerts P, Verbeke K (2007) Effects of Lactobacillus casei Shirota, Bifidobacterium breve and oligofructose-enriched inulin on colonic nitrogen-protein metabolism in healthy humans. American Journal of Physiology 292: 358-368.

Objective

To investigate the effect of prebiotic (oligofructose-enriched inulin; OF-IN) and/or probiotic (Lactobacillus casei Shirota, LcS; Bifidobacterium breve Yakult, BbY) on proteolytic fermentation by colonic bacteria.  

Methods

The 16 week crossover trial involved healthy volunteers (n=20) randomised to two treatment groups. The twice daily, four week interventions were a probiotic fermented milk drink containing LcS, a lyophilised probiotic powder of BbY, the prebiotic OF-IN or their corresponding placebos, with each intervention separated by a two week washout period. An additional intervention for one of the groups was the synbiotic combination of LcS and OF-IN.

To analyse the effects of this on colonic protein metabolism, a test meal containing a stable isotope of lactose (lactose[15N, 15N]ureide), was consumed at baseline, at the start and end of each intervention and during the washout periods. Urine samples (taken at baseline and 48 h after consumption of test meal) and stool samples (collected during 72 h after the test meal) were analysed two biomarkers: 15N and p-cresol. (Full details of the analysis are given in the paper).

Results

Prebiotic intervention resulted in a significant decrease in urinary excretion of 15N after 48 h and 4-weeks compared to baseline (P = 0.005 and P = 0.017 respectively) and to the placebo group (P = 0.005 and P = 0.022 respectively).

After 48 h intervention with LcS, a significant reduction in 15N excretion was observed (P = 0.038). There was also significant reduction in 15N excretion with the synbiotic intervention after 48 h and 4-weeks compared to baseline (P = 0.008 for both terms) and to the placebo group (P = 0.011 and P = 0.008 respectively).

Both probiotics resulted in significant decreases in p-cresol excretion compared with baseline (BbY: P = 0.028 after 48 h; P = 0.005 4-weeks. LcS: P = 0.038 after 4-weeks). The same was observed with the prebiotic (P = 0.001 after 48 h; P = 0.005 after 4-weeks) and synbiotic (P = 0.021 after 4-weeks) interventions.

Conclusions

Dietary interventions with prebiotic or probiotic (oligofructose-enriched inulin, L. casei Shirota, B. breve Yakult) favourably reduced colonic protein metabolism.

 
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